Hello all,
I need some help understanding the agilent application note: Quantification of THC and CBD in Gummies and Hard Candies, specifically where the dilution factor is calculated
The SOP states:
1g gummy into 10ml water, then homogenise
add 10ml ACN to solution, homogenise
add quechers salt, shake + centrifuge
top 10ml ACN layer contains analytes
remove 2ml of the supernatant.
filter into sample vial
when i use x10 DF factor i am getting really high numbers which dont add up. Can anyone explain where the DF x10 comes from? I emailed one of paper authors and he just repeats that its x10. his reply was "You have a good question. The dilution is indeed 10X because you finish in 10 ml acetonitrile. In other words, you go from 20ml to 10 ml." but surely going from 20ml to 10ml, is a dilution factor of 0.5
im really confused, please help. i typicall use final-volume/initial-volume (or aliquot) to determine DF which seems to work, except in this example
Tan