i have a 95% purity liquid sample (i would call this COMPOUND A), with an average polarity.
I have in it this 2% impurity (I would call it COMPOUND B) , less polar.
What kind of SPE I can use to separate this?
My sample A is soluble in methanol, hexane, acetone, acetonitrile but not in water.
I can separate the two compound using hplc C18 , isocratic WATER/METHANOL 25/75 :
-compound A leave the column at rt 15 min
-compound B leave the column at rt 37 min
I want to try SPE just to separate the two compound at atmospheric pressure....ok using a little only a little pressure.