I ordered the QuickChange Site Directed Mutagenesis Kit - Catalog # 200524 - 5. I have been unable to achieve site directed mutagenesis with your kit with 1μl dNTP as stated in the Instruction Manual but need at least 3μl to achieve mutagenesis.
I ordered the QuickChange Site Directed Mutagenesis Kit - Catalog # 200524 - 5. I have been unable to achieve site directed mutagenesis with your kit with 1μl dNTP as stated in the Instruction Manual but need at least 3μl to achieve mutagenesis.
Please contact techservices@agilent.com for help to analyze this issue and achieve a solution.