Inter detector delay triple detection

The phenomenon you describe is inevitable for broad polymer samples: The signal intensity (SI), of all detectors depends on injected mass (concentration and injection volume), a sample related constant k_Sample (i.e., response factor), and a detector constant K_Detector. Additionally, some detectors generate signals that are dependent on the molar mass of a sample.

All detector signals follow the same equation: SI = K_Detector · k_Sample · c · M_x
SI = signal intensity
K_Detector = detector constant
k_Sample = sample constant or response factor
c = concentration
M = molar mass

The exponent x divides detectors into two categories:
1) Concentration detector, x = 0
2) Molar mass sensitive detectors, x ≠ 0 (LS detector x = 1; Visco detector x = α)

Since constants and the influence of molar mass differ for concentration and molar mass-sensitive detectors, each detector yields a different signal output for a given sample. This difference is more pronounced for broad distribution samples. Please read more about GPC/SEC detection in the source (linked eBook) to my answer. Check further eBook material in the Wiki.

The phenomenon you describe is inevitable for broad polymer samples: The signal intensity (SI), of all detectors depends on injected mass (concentration and injection volume), a sample related constant k_Sample (i.e., response factor), and a detector constant K_Detector. Additionally, some detectors generate signals that are dependent on the molar mass of a sample.

All detector signals follow the same equation: SI = K_Detector · k_Sample · c · M_x
SI = signal intensity
K_Detector = detector constant
k_Sample = sample constant or response factor
c = concentration
M = molar mass

The exponent x divides detectors into two categories:
1) Concentration detector, x = 0
2) Molar mass sensitive detectors, x ≠ 0 (LS detector x = 1; Visco detector x = α)

Since constants and the influence of molar mass differ for concentration and molar mass-sensitive detectors, each detector yields a different signal output for a given sample. This difference is more pronounced for broad distribution samples. Please read more about GPC/SEC detection in the source (linked eBook) to my answer. Check further eBook material in the Wiki.