Cannula usage

Hi, What is the recommendation for using cannula in manual sampling?
How it can be assured that the cannula is clean and dry inside?
At which point the cannula should be inserted into dissolution vessel?
Should the cannula be primed in any way before the final sample is pulled?
Can you provide a technical opinion on the risk of “contamination” or unrepresentative sampling that could occur if we are taking multiple samples from the same vessel (i.e. 5min, 10min, 15min) using the same cannula?
Is the sampling procedure the same when using cannula filters and syringe filters?
Thank you

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