Hi,
I need to analyse phosphate and glucose in the same analysis on HPLC I have tried using different columns such as Aminex HPX-87H Column 300 x7.8 mm, Agilent Hi-PLex H, 7.7 x 300 mm, NUCLEOGEL SUGAR 810 H, 300x 7.8 mm... but none of these manage to separate the two peaks which remain superimposed... does anyone perhaps know of a different column to use or suggestions for parameters considering the analytes? Or any suggestions considering the properties of the compounds ?
Best,
Chiara