We have an issue of peak shape deterioration during the analysis(Column details: Zorbax Eclipse plus C18 4.6 x 100 x 3.5µm) Pressure was constant throughout the sequence.
Tried with other eclipse plus column with same dimensions, but we got the same peak deterioration after every 3 or 4 sequences.
We use dedicated columns for each product.
Mobile phase details: Potassium Dihydrogen phosphate buffer at pH 8.5(Mobile phase-A and acetonitrile(Mobile phase-B)
Column temperature: 40 °C. Filtered the Mobile phase with 0.45µm PVDF membrane filter.
Column washing post sequence with 80:20 Methanol and water.
Kindly suggest recommendations to improve the peak shape and column.