I am currently using Preparative LCMS to purify my sample (around 150 mg). The column type is Part Number:990967-202 (ZORBAX Eclipse XDB 80Å C18, 9.4 x 250 mm, 5 µm HPLC column)
I have tried a 0.1mL injection of 30mg/mL sample (3mg sample load) and it worked fine.
However, I am not willing to separate my samples dozens of times whilst I am a bit concerned about blocking the column if I inject more than it can accommodate (i.e. 30mg in 1mL).
So, I would really appreciate it if someone could help me with this obtaining the information on the maximum sample load and concentration for the following column, please?