Detection limit

I want to determine low molecular organic acid in HPLC using Agilent 1290 infinity ii UV detector. Can anyone say what is the detection limit of that instruments? Thanks

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  • Hi tamjid,

    a detection limit is dependent on multiple factors: detector type, analyte structure, detector cell volume, injection volume, internal volume of your tubings, system noise, retention time, peak width, etc. Therefore we cannot just predict a limit of detection and you have to do this the textbook way.

    Regards,

    Norbert 

Reply
  • Hi tamjid,

    a detection limit is dependent on multiple factors: detector type, analyte structure, detector cell volume, injection volume, internal volume of your tubings, system noise, retention time, peak width, etc. Therefore we cannot just predict a limit of detection and you have to do this the textbook way.

    Regards,

    Norbert 

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