without knowing the type of contaminant it is very difficult to give you a cleaning procedure.
The general procedure would be reversing the column and using your solvent (THF in this case) at 50% of the normal column flow you use for ca. 20 column volumes.
If this does not help you could add some percentages of NMP or DMSO or DMF to the THF and do the same, but pay attention to the system pressure as those solvents have a higher viscosity and therefore give you more pressure, better to lower the flow then.
without knowing the type of contaminant it is very difficult to give you a cleaning procedure.
The general procedure would be reversing the column and using your solvent (THF in this case) at 50% of the normal column flow you use for ca. 20 column volumes.
If this does not help you could add some percentages of NMP or DMSO or DMF to the THF and do the same, but pay attention to the system pressure as those solvents have a higher viscosity and therefore give you more pressure, better to lower the flow then.
We are glad this was helpful! We are sorry this was not helpful. If you still need assistance please create a community post or contact support. To help us improve, please provide any additional feedback.
For full details of how we will treat your information, please view our privacy policy.
