If it is in a "simple matrix" like wine a Bond Elut C18 (500 mg or 1000 mg) would work:
Conditioning Step:
2 X 3 mL acetonitrile, 2 X 3 mL H2O
Sample Application:
100 mL red wine applied to 1 g column 100 mL white wine applied to 500 mg column 0.1 ug/L spiked sample applied to 5 g column
Elution Step:
2 mL acetonitrile Spiked sample: 6 X 1 mL acetonitrile
Eluent Handling:
Evaporate to 0.4 mL under N2
Remark:
Least concentrated samples required highest cartridge capacity due to great concentration of compound which compete for the binding sites. When OTA is in high concentration there is no retention problems found
If it is in a "simple matrix" like wine a Bond Elut C18 (500 mg or 1000 mg) would work:
Conditioning Step:
2 X 3 mL acetonitrile, 2 X 3 mL H2O
Sample Application:
100 mL red wine applied to 1 g column 100 mL white wine applied to 500 mg column 0.1 ug/L spiked sample applied to 5 g column
Elution Step:
2 mL acetonitrile Spiked sample: 6 X 1 mL acetonitrile
Eluent Handling:
Evaporate to 0.4 mL under N2
Remark:
Least concentrated samples required highest cartridge capacity due to great concentration of compound which compete for the binding sites. When OTA is in high concentration there is no retention problems found
We are glad this was helpful! We are sorry this was not helpful. If you still need assistance please create a community post or contact support. To help us improve, please provide any additional feedback.
For full details of how we will treat your information, please view our privacy policy.
229 Ochra 