What combination of substrate I need to use on my isolated mitochondria on seahorse plate to keep them respiring?

Thanks Courtney,

I went through the recommended papers, and it helped me to understand better. What I could not find that what combination of substrate I need to use on my isolated mitochondria on seahorse plate to keep them respiring? I am using seahorse assay buffer (DMEM+Sod. Pyru+Gluc+Glutamine) for the cells to be used in the assay. 

My set-ups are:

Port-A: ADP (Different concentration from 0.5 to 4 mM)

Port-B: Oligo

Port-C: FccP

Port-D: Rot+Antimycin

I am using primary neurons from the mice to isolate the mitochondria.

Let me know!

Best,

Gaurav

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