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Table of Contents
  • AssayMAP Resources
  • AssayMAP Protein Sample Prep Workbench 3.0 Release
  • AssayMAP Protein Sample Prep Workbench v3.1 Release
  • AssayMAP Protein Sample Prep Workbench v3.2 Release
  • Maximum volume transfer of a 250ul tip on an AssayMAP head
  • AssayMAP User Group Talks Cambridge, MA
  • Virtual AssayMAP Bravo User's Group Meeting
  • North American Virtual AssayMAP User Meeting Recording
  • ASMS AssayMap Bravo User's Reception
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AssayMAP Resources

Created by mnevill mnevill over 2 years ago | Last modified by mnevill mnevill over 2 years ago

 

 

AssayMAP Resources

Videos

  • AssayMAP Bravo Tutorial Session
  • Automated Protein & Peptide Sample Preparation
  • Virtual AssayMAP User Meeting on demand

App Notes

  • Human *** Cancer Cell Line Phosphoproteome Revealed by an Automated and Highly Selective Enrichment Workflow
  • Host Cell Protein Analysis Using Agilent AssayMAP Bravo and 6545XT AdvanceBio LC/Q-TOF
  • A streamlined drug-to-antibody ratio determination workflow for intact and deglycosylated antibody-drug conjugates
  • An Integrated Workflow for Intact and Subunitsof Monoclonal Antibody Accurate Mass Measurements
  • Automation of Sample Preparation for Accurate and Scalable Quantification and Characterization of Biotherapeutic Proteins Using the Agilent AssayMAP Bravo Platform
  • Accurate and Scalable Quantification and Characterization of Biotherapeutic Proteins using AssayMAP Bravo
  • Determination of Drug-to-Antibody Ratio for Antibody-Drug Conjugates Purified from Serum
  • Workflow Automation for LC/MS: In-Solution Protein Digestion, Peptide Cleanup, and Strong Cation-Exchange Fractionation of Peptides Enabled by AssayMAP Technology
  • Automation for LC/MS Sample Preparation: High Throughput In-Solution Digestion and Peptide Cleanup Enabled by the Agilent AssayMAP Bravo Platform
  • Agilent AssayMAP Bravo Technology Enables Reproducible Automated Phosphopeptide Enrichment from Complex Mixtures Using High‑Capacity Fe(III)‑NTA Cartridges
  • A streamlined drug-to-antibody ratio determination workflow for intact and deglycosylated antibody-drug conjugates
  • Rapid Antibody Digestion Enabled by Automated Reversed-Phase Desalting on the Agilent AssayMAP Bravo Platform
  • An Integrated Workflow for Automated Calculation of Antibody‑Drug Conjugate (ADC) Drug‑to-Antibody Ratio (DAR)
  • An Integrated Workflow for Intact and Subunits of Monoclonal Antibody Accurate Mass Measurements

Flyers

  • Agilent AssayMAP Workbench v3.0

New Software Updates

  • Agilent AssayMAP Workbench v3.2
  • Agilent AssayMAP Workbench v3.1
  • Agilent AssayMAP Workbench v3.0

Webinars

  • High Throughput Glycan Analysis of Monoclonal Antibodies
  • Proteomics Research (Podcast)
  • Targeted Proteomics Assays for Cellular Signaling Analysis

Quick Start Guides

  • Affinity Purification
  • Fractionation
  • IMAC Cartridge Customization
  • Immobilization
  • In-Solution Digestion: Single Plate
  • In-Solution Digestion: Multi-Plate
  • Normalization
  • On-Cartridge Reaction
  • Peptide Cleanup
  • Phosphopeptide Enrichment
  • Protein Cleanup
  • Reformatting Utility
  • Serial Dilution Utility

User Guides

  • Knowledge Base
  • Antibody Purification Protocol Guide
  • Affinity Purification
  • Fractionation
  • IMAC Cartridge Customization
  • Immobilization
  • In-Solution Digestion:Multiplate
  • In-Solution Digestion:Single-plate
  • Normalization
  • On-Cartridge reaction
  • Peptide Cleanup v1.1
  • Peptide Cleanup v2.0
  • Phosphopeptide Enrichment
  • Protein Cleanup
  • Reformatting Utility
  • Serial Dilution Utility

Troubleshooting & Misc

  • Error Recovery Guide
  • Labware Reference Guide
  • Syringe Replacement Guide
  • Wash Station Maintenance Guide
  • AssayMAP Bravo Platform Installation Guide
  • Cartridge Selection Guide

 

  • bioprocessing
  • automated sample prep
  • bioprocess optimization
  • assaymap
  • automated
  • sample preparation
  • immunoprecipitation
  • drug discovery
  • affinity purification
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  • smurphy
    smurphy over 2 years ago in reply to jashnaniaarti

    Hi Aarti,

     

    Our standard recommendation is to wash the syringes with 250 µL of 50 mM NaOH using the Syringe Wash utility between every run.  This works well for cleaning the syringes the majority of the time. 

     

    If you experience run-to-run carryover that is not resolved by washing with 50 mM NaOH, the Syringe Wash utility allows one to wash with up to 6 different solutions which can cover a wide range of chemical space.  For example, one can do high ionic aqueous washes at high and low pH,  organic washes at high and low pH wash, and/or denaturing reagents (e.g. urea or guanidine) washes. 

     

    In the extremely rare cases where the approaches above do not resolve the issue, we have a couple of other options that can be tried but the approaches above address carryover in all but the most extremely "sticky" proteins.  If you do run into this issue, please contact me or technical support to discuss other options. 

     

    Steve Murphy

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  • jashnaniaarti
    jashnaniaarti over 2 years ago

    Hello,

     

    I am looking for a syringe clean-up protocol for when we use serum samples. DO you have any recommendations?

     

    Thanks,

    Aarti

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  • nswayne
    nswayne over 3 years ago

    Thank you!

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