This Information Applies To: Agilent OpenLab CDS (Rev. 2.x)
This article outlines the step-by-step instructions on how to extract a UV spectrum with the Spectrum Extraction Tool.
If your data contains UV spectral data, you can extract spectra from your chromatogram. You can either:
The behavior of the spectrum extraction tool is configurable. To change the behavior, click in the Chromatograms window, and adjust the settings in the Spectrum extraction section. In the following procedure, all options are enabled.
Figure 1: Store All Spectra setting in acquisition method for DAD
Figure 2: 3D UV Quantitative Processing Method
Figure 5: Confirmation dialog box at peak apex
Figure 6: Confirmation dialog box at selected time point.
The extracted spectrum is shown in the UV Spectrum window.
The time point or time range used for subtraction is shown in the spectrum name.
The extracted spectrum is listed under the details for the individual injection in the injection tree.
You can also extract UV spectrum via a right clicking a peak or extract multiple UV spectra. See articles How to Extract a UV Peak Spectrum Via Right Click in OpenLab CDS and How to Extract UV Spectra From Multiple Peaks in One Step in OpenLab CDS
Figure 7: Extract a UV spectrum using the Spectrum Extraction Tool
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