Setting Gain for Measuring Fluorescence Using Biotek Synergy H1 Microplate Reader

Question

I am using a Biotek Synergy H1 microplate reader and the Gen5 software version 3.12.8. 
 I have been trying to measure fluorescence using the machine, but I am having trouble setting the gain. 
 The default shows <extended> gain and does not allow me to set it to a particular value. When I produce reports, it shows that the gain was zero and sometimes 100. How do I set it so that I can manually put the value?

hjermd · Answer

Hi and thank you for the question! 
 To switch from extended dynamic range to standard range which allows a manual setting you need to click on the edit button next to the read speed selection and switch to "standard". 
 Once you are using standard dynamic range you will be able to type in a gain value within the allowable working range. 
 Typical gain settings-

Detection 
 Type 
 Low 
 High

Fluorescence 
 Filters 
 35 
 120

Fluorescence 
 Monochromator 
 50 
 150

Luminescence 
 Filters 
 100 
 255

Luminescence 
 Luminescence fiber 
 100 
 255

Time-Resolved FL 
 Filters 
 100 
 255

Time-Resolved FL (Neo2 Laser) 
 Filters 
 75 
 255

Time-Resolved FL: Synergy HT 
 Filters 
 150 
 255

Fluorescence Polarization 
 Filters 
 35 
 120

Setting Gain for Measuring Fluorescence Using Biotek Synergy H1 Microplate Reader

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