I have been analyzing some polymers with a more polar solvent on SEC - ELSD detection. (relative to Polystyrene)
Where I normally measure a MW of 30K for PLA (relative to Polystyrene) in chloroform, I now measure a MW of 140K.
In other words, in this polar solvent, the PLA has a bigger hydrodynamic volume, right?
If the solvent surrounds the polymer more, and this causes a bigger hydrodynamic volume, would the analysis then be incorrect?
Let me know what you think,