Peak Broadening after flushing column with isopropanol



Is it normal to see the peaks broadening after flushing with isopropanol? This is the second time we flushed the column with isopropanol. The first time also gave the same peak results and therefore we changed to a new column. Now with the new column, we tried to clean the column again with isopropanol and then we run our sample with our solvent and the peaks broaden. Any suggestion on how to solve this?  We are using:


Column: Phenomenex Luna 3um silica,

Mobile phase: Hexane:acetone


Thanks for your help.

  • Hi,

    unfortunately we cannot give you advise about a competition column as we do not know about their quality. Washing a column with IPA should be possible, but afterwards you need to slowly add organic modifier and then replace IPA by water to bring RP column back. Unfortunately again, there is no guarantee that it works as it is unknown to us what you did with the column.



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