Anyone using the Enhanced Matrix Removal – Lipid kits

Question

I am looking to try using the Enhanced Matrix Removal – Lipid kits to prepare Vegetable and Fish Oil samples for PAH testing via GC. Looking for anyone who has used these for advice.

Agilent | Enhanced Matrix Removal – Lipid

alexucci · Accepted Answer

Hi Avrom, While we do not have any dedicated application notes yet for oils, this would be a good starting protocol to try: Extraction step: 2 grams of oil to 10 mL of acetone Clean-up step: Add only 1 mL of water to activate the EMR-Lipid material (pn# 5982-1010) (vortex immediately/aggressively) Then add 5 mL of extract from the extraction step to the EMR tube (vortex/centrifuge) "Polish Step" (to remove water and any dissolved solids) Decant from the EMR-Lipid tube to the "Polish Tube" (5982-0101) (vortex immediately and centrifuge) Because you are performing GC analysis, we recommend performing an extra dry step to remove any residual water. This involves using 300 mg of MgSO4 per 1 mL of extract. We offer pouches of 3.5 g of anhydrous MgSO4 (5982-0102). Transfer desired amount of extract to an empty centrifuge tube with the appropriate amount of MgSO4. Vortex immediately and centrifuge. At this point, you can transfer to an autosampler vial or perform any dry-down/reconstitution steps. Because your sample is an oil, 2 grams of oil would be a good starting point. Anything above that may over saturate the EMR-Lipid material. I will be reaching out to you directly to discuss more about the product offering. Thank you -Alex