QuikChange Lightning Site-Directed Mutagenesis Kit

Question

Is the &ldquo;QuikChange Lightning Site-Directed Mutagenesis Kit&rdquo; above the same component as part of the mutagenesis reagents in the QuikChange HT Protein Engineering System (Part Number:G5901B)? The name of some of the reagents and the reaction volume are similar so I wonder if we can adapt the &ldquo;QuikChange Lightning Site-Directed Mutagenesis Kit&rdquo; in our lab with our own mutation oligo library. Thanks!

agrafsky · Accepted Answer

While the names of the products and their reagents appear similar, there is a (proprietary) qualitative difference between them. As a result, the QuikChange Lightning product is more suited to the generation of a single mutation within a targeted sequence using a particular pair of primers. Alternatively, the QuikChange HT product is more suited to the generation of a library of mutagenized sequences using a group of custom-designed primers. Consequently, in order to generate optimal results, it is not recommended that the reagents from one product be substituted within the protocol of the other.

QuikChange Lightning Site-Directed Mutagenesis Kit

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