We are having a problem with our semi-quant single-point calibration curve for THC Analysis. We are not seeing a response factor of the calibration curve after inputting the qualifier ions for our internal standard OR our unknown THC samples. Our qualifiers are m/z: 299, 231, 314 and m/z: 302, 234, and 317. This is the first time we have seen this problem. We have recently changed the column and placed a new filament into our EI source detector. We are using a MSD ChemStation F.01.03.2357.