AnsweredAssumed Answered

How to inject different volumes indicated in sequence?

Question asked by danielchsny on Sep 24, 2020

Hi, I am working on an LC-MS single quad 6120b

Running Open Lab CDS Chem Station. When I set a sequence there is a column called: 'injection volume'. If I set different volumes in different rows I expect to inject different volumes. However, the same volume is injected for all samples. It looks the autosampler method 'corrects' the volume.

 

Funny, during the injection, the drawing indicates that a different volume is injected. However, in .d/acq.txt shows the autosampler method volume. Analysis of the data also shows the same volume was injected for all samples.

 

Is there a way to set a sequence with different volumes?

 

thanks

Outcomes