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Poor Resolution with High Concentrations

Question asked by aflalab on Aug 25, 2020
Latest reply on Aug 31, 2020 by aflalab


We notice that when analyzing our calibration standards, the resolution between peaks decreases as the concentrations increase. We are using a Microsorb-MV 100 C18 250 x 4.6 mm 5um column @ 1.000mL/min and 5uL sample volume. How can we improve resolution for samples with higher concentration?