Hi, I would like to know how activate indirect detection in Chemstation version B.0301 (317) for calcium analysis in HPLC using chromatographic column Hamilton PRP-X800
If You are using a DAD set reference wavelength as the signal and the max. wavelength of your background electrolyte as background for subtraction -
in CE for example when using cations with PAP as the background - see https://www.agilent.com/cs/library/applications/5990-5244EN.pdf
Detector: Signal 240 nm, reference: 210 nm, Bandwidth: 10 nm
For the method using 2mM Cupric Sulfate (Isocratic. Ambient.
0.8 mL/min. 10 μL, Indirect UV 220 nm) check the UV spectrum of Cupric Sulfate and invert the Spectrum (maximum becomes reference, as signal use the closest minimum next to max)
For a VWD change polarity (https://www.agilent.com/cs/library/usermanuals/Public/G1314-90013_VWD-B-C_USR_EN.pdf)
Retrieving data ...