I did the tuning as regular day. Then I tried to calibrate the instrument but all the elements on the no gas mode are out and they don't have any counts!!
Are you using multitune in your batch and if so, is the no gas tune the first tune used? If so, perhaps the sample isn't reaching the nebulizer in time for the analysis. I would check your sample uptake settings and your peri-pump tension if applicable.
Thanks a lot, yes the problem was peri pump setting. Now all is good.
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