I can see the calibration peaks but nothing appears on the curve
Please provide more detail.
What software product (OpenLab CDS, OpenLab ChemStation, OpenLab EZChrom, etc...) are you using? What revision (found by going to either Help>About or File>About)?
Have you specified calibration standards in your sequence table/injection list and reprocessed after your method was setup in order to build a calibration table? What steps have you performed so far?
On closer inspection it appears that the software is trying to use data from a previous run for one of the calibrants, not the run that was just executed?
On the break down of the calibration it is using two points from X calibrant which was previously done, and 1 of the two points from calibrant X form the current run. A bit confusing sorry
I am using OpenLab CDS.
Could you please provide screenshots identifying the issue? I am not sure I am understanding the problem you are describing.
In OpenLab CDS you should just need to identify the calibration standards in the sequence table/injection list by selecting a Sample Type of Cal. Std. Then you will want to Clear the calibration history points (if you do not want previous calibrations to weight the calibration curve) and specify a calibration level for each Cal. Std. injection:
If a previous run is being used in the calibration curve then you are probably not clearing out the old calibration by either selecting 'Clear all calibration' for the first cal std of the sequence or 'Clear calibration at level' for the first injection of the cal std for each level.
I have removed the old calibration using what you suggested above, thank you. However I still can't get a curve to show.
Images attached hope these help
Thank you for the screenshots. Those clear things up a bit. You are referring to the GPC column calibration, not a compound calibration curve which is what I assumed you were asking about.
I have attached the User Guide for the GPC OpenLab CDS add-on. Starting on page 23 it outlines ho to generate a GPC Column Calibration. I recommend you review those instructions and then let us know if you are still running into problems.
Retrieving data ...