I used the no injection instrument blank with a gradient method and was expecting the method to run through the changing mobile phase gradient but it stayed at the gradient starting conditions. Is this to be expected?
I'm using Openlab 2.3
The response from Agilent's Informatics team is that the "no injection/instrument blank" is not supported by the Alliance
driver. This applies to all CDSs. This is planned to be addressed in Alliance driver v6.3.0 scheduled for the end of 2020.
The workaround suggested is to use an injection volume of 0.
My thoughts are - what extra validation will be required if the driver is updated?
The suggested workaround doesn't emulate the no injection/instrument blank as the autosampler will not be bypassed when using an injection volume of 0. Please correct me if I'm wrong.
A No Injection/Instrument Blank should just skip the autosampler's portion of the method and run through the rest of the method. What firmware do you have loaded on your instrument's modules and what version of the Agilent LC driver do you have installed?
The firmware is 2.04 and the LC driver is Waters Alliance 6.1.4. (We are using a Waters Alliance 2695)
Thank you for the information. I am unfamiliar with the Waters Alliance instrumentation and firmware so I cannot weight in on this. I did a cursory search of the logged defects in OpenLab CDS and did not find any that line up with your problem description.
OK thanks. I will verify the issue and then log a call with Agilent customer services.
Thanks for looking into it.
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