What SPE cartridge to use? ochratoxin A , similar to OchraTest WB Vicam or MycoSep® 229 Ochra
what to use is strongly dependent on the matrix the mycotoxin is in. We have several application notes dealing with OTA:
This is just a selection.
If it is in a "simple matrix" like wine a Bond Elut C18 (500 mg or 1000 mg) would work:
2 X 3 mL acetonitrile, 2 X 3 mL H2O
100 mL red wine applied to 1 g column100 mL white wine applied to 500 mg column0.1 ug/L spiked sample applied to 5 g column
2 mL acetonitrileSpiked sample: 6 X 1 mL acetonitrile
Evaporate to 0.4 mL under N2
Least concentrated samples required highest cartridge capacity due to great concentration of compound which compete for the binding sites. When OTA is in high concentration there is no retention problems found
Retrieving data ...