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Rinsing HPLC system

Question asked by bendopharma on Jun 10, 2020
Latest reply on Jun 11, 2020 by bendopharma

Hello,

 

We mostly rinse the column and the system after the analysis with 70% acetontril in water.

So this will be in the HPLC system.

My question is if it doesn't matter when we then use a mobile phase with fosfate buffer or do we first have to rinx=se the HPLC system with a more aqueous solvent such as 50% methanol in water.

 

Thanks,

Ben

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