EIC are flat-lined where peaks are expected. Probably something simple but I'm at a loss how to proceed. TIA!
Could you share the EIC and spectrum of the analyte RT? We would have But there might be thousands reasons why you do not have a peak.
First, you should extract accurate mass, with 3 or 4 decimal places. Nominal mass will not work for HRMS. You can change extraction window width at the "extract chromatogram", where you set an extracted mass. It is usually in ppm, I would recomend 20 or 40 ppm.
Even better is to use "Find-by-formula", where you paste formulas of your analytes and it will automatically calculate exact mass of different adducts, extract them and qualify also isotopic pattern.
There still might be a problem with a mass accuracy (shifted masses) or something else. But exact masses are essential.
Well, no wonder.
However, now I can't seem to get a passing calibration.
Now I've lost the TIC.
Retrieving data ...