I have run some samples and then I did the calibration table.
The table I have made does not give me a concentration for my compound. So, how could I use it for the integration of my compound?
Thank you in advance
Please provide us with the name of the software product your are using (OpenLab ChemStation, EZChrom, MassHunter, etc...) as well as the revision. You should be able to determine both of these by going to either Help>About or File>About.
I am using """"" OpenLab CDS/ Chemstation Edition/ For LC&LS,MS Systems """"""
The revision is : """" C.01.09 (144) """"""
The problem is that I have run my samples and then I want to do the calibration now.
I know how to make the calibration table.
After that I should go to Report >>>> Specify Report >>> Quantitation Settings >>>> Calculate: ISTD>>>> Area or Height>>> OK>> Save method ( This is not activated in my software and I can not save my method.) >>>> Sequence Table>>> SAmple Type: Calibration>>>>> Cal level: 1, 2, 3, .....>>>>>Update RF: Replace >>>>> Reprocess.
But I do not know how to use it for quantitation of my compound.
I actually have set a method that I run my samples with that. When I integrate, it gives me a table including AREA of my analyte and IS .
I have done it through EXCEL. I made the calibration curve and based on my analyte and IS area and also the concentration of my IS which is always similar and with help of calibration curve I can find the concentration but I would like to learn how I can also find the concentration in the software.
I really appreciate it if you reply to my email ASAP since I am running another experiment in which I need my previous DATA.
Thank you so much for your help in advance
If you have a calibration table saved to your method and you have reprocessed the data with said method then ChemStation will automatically calculate an amount using the response factor calculated from the calibration curve. As long as the compounds you have identified in your calibration table are present in your samples and you are using an appropriate report template then amounts should be included on your reports.
Just to make sure you are setting up your calibration table correctly, here is a link to a video outlining how to setup an ISTD calibration curve in ChemStation: How to create an Internal Standard Calibration Curve in OpenLAB CDS Chemstation
Once this is done and the data has been reprocessed, you should be able to use just a classic short report or the default ISTD intelligent report template. To select the report template you want to use with you method just go to Report>Specify Report. This is also where you select a destination for the report you generate (screen, printer, file). To print the report for an injection you can then go to Report>Print Report.
Thank you so much for your help. I have actually used the video you sent to me for doing the calibration. My calibration curve is ready now.
So, when I finish calibration, I should select the folder including the samples I ran before and then Report>>> Specify report>>> and choose classic reporting>>> and choose the file in Destination part. Is that right?
I did not find what is report template. Could you please clarify?
Thank you so much for your assistance
If you want to use classic reporting then yes, those steps will work. You just need to select the type of reporting (intelligent vs. classic), the report style (for classic) or template (for intelligent), and the report destination. Once those are selected and saved to the method, the report will be printed/generated when each injection is processed. To generate just a report for the currently loaded injection you can go to Report>Print Report. If you want to generate injection reports for all injections in a result set then you can reprocess the result set.
I have done all the process as you described in your email. However, it did not work.
I have recorded a video to show how I do it. I have attached it for you. Could you please take a look?
Thank you so much
I am unable to play your video attachment. I would recommend you contact your local Agilent support if you have not done so already: Contact Us | Agilent
I have also another question. I have the highest peak in one uv absorbance while this high peak is appeared for my IS with another Uv absorbance.
I would like to know for quantitation, may I use both uv absorbance? I mean for example 215 for my IS and 254 for my analyte.
Thank you so much
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