AnsweredAssumed Answered

Tapered syringe needle increases abundance?

Question asked by vadfs1 on May 4, 2020
Latest reply on May 5, 2020 by smathers

Why do my sample abundances increase X3 using a tapered 23/26 gauge syringe  vs a straight 23gauge syringe? I did watch to be sure it was delivering 1uL of sample. My values increased from just over 300 to over 1000 with the change in syringe.

 

According to this table, the internal diameter of the 2 gauges are the same.   Even if the internal diameter of the smaller bore were smaller, how would this increase the amount of sample introduced onto the column?  

 

 

Running on a 7890GC.

 

Syringe Part #'s

23/26gauge tapered Agilent 5181-1267

23 gauge straight Thermo 365D1576

Outcomes