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Negative peak

Question asked by marcemexico on Apr 17, 2020
Latest reply on Apr 18, 2020 by marcemexico

Good afternoon


I am working with tuberous root to know monosaccharides, the sample does not have any types of processing ( enzymatic hydrolysis). An Agilent 1260 Infinity with IR detector and Agilent Hi-Plex H ( 7.7x300 mm, 8 um) column was used. My doubt arises as a negative peak is observed in the chromatogram and this first peak indicates is very small and marks an area percentage of 30.224.

In the chromatogram reporte it does not indicate any negative number. Someone could guide me on what is happened or what is causing this peak. I attach chromatogram report.

 

Chromatogram

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