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Clog in HPLC

Question asked by lmccracken on Mar 17, 2020
Latest reply on Mar 18, 2020 by lmccracken

I believe I have a clogged up HPLC (Agilent 1260 infinity II) due to sample precipitating while running.  Its not in the column, I believe, but I think it's clogged in a line somewhere before the column.  If I try to flush the lines at only 0.1mL/minute, the pressure is getting so high the entire instrument is stopping.  What is the best way to determine where this clog is....removing line by line to see if the flow will start again?  I've only had this happen one other time and it was easily cleaned...this one is not.  Please advise if there is a good place I can go to read about cleaning and best way to get rid of the clog?  Thanks!