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Molecule (melatonin) won't ionize with ESI, has anybody else run into this problem?

Question asked by kebattaglia on Mar 10, 2020

I'm trying to identify melatonin and seratonin.  When I run the standards, each one has it's respective peak but it's the molecular parent ion, not a fragmentation that shows up.  I'm currently using ESI as the ion source, but it seems like APCI may be a better fit given the problems I've run into. Before purchasing the APCI ion source, I want to know if there are other ways to trouble shoot this issue, with changing parameters, etc.  Thank you! 

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