I need to transfer a UPLC method to HPLC...can anyone help me understand how to convert?
Can you give us more information about the method that you want to transfer. Going from UHPLC methods to HPLC isn't always possible, especially for gradient methods, due to the large differences in volumes of the different types of hardware involved.
Thank you! I struggled to get this one, and it didn't work out so I'm switching to a normal HPLC column. Thanks again!
There is a free course on Agilent University that covers HPLC to UPLC method transfer:
HPLC-1290-2190r - 1290 Infinity LC Systems - Method Transfer HPLC to UHPLC
I know that you are trying to go the opposite direction, but it may still be helpful.
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