in LC-MS, i was wondering how does negative ionization (Negative Mode) work with peptides?
Same sample has shown differece in the intensity of the ionization, it is much lower in negative mode .. the negative is super important for me because it is much more clear.
Should i increase the voltage? if so.. to what value?
my mobile phase is TFA, is it possible that TFA iones interfere and that is why the negative ioniztion is weak?