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What is the cause of a shift in the FLD signal during a run?

Question asked by mvsteenl on Jan 14, 2020
Latest reply on Jan 15, 2020 by sschombu



We often run amino acid analysis with a HPLC FLD (G1321A). But recently we have some problems with our chromatograms.


For the detection of primary and secondary amino acids, a different excitation and emission wavelengt is needed. So for the analysis a timetable for the FLD signal is set up:


Timetable FLD


Recently we have noticed that, at 17,40 minutes, when the FLD switches its signal, it also causes a shift of the baseline in our chromatogram. Which means we are not able anymore to detect our secondary amino acids.



Does anybody has expercience with such a problem?


Thank you!