We have a an aldehyde that we are derivatizing with 2,4-dinitrophenyl hydrazine (DNPH). Literature searches suggest the resulting derivatization compound produces two isomers; one cis and one trans. These two compounds elute at different times as different peaks; their total area representing the total amount of aldehyde present in the sample.
The ratio of the area of these two peaks does not remain perfectly constant, so it's not possible to create an accurate calibration curve based off just one of the peaks.
We need to be able to add the area of the two peaks together and then build a calibration curve off of that summed area. This seems like something that would be built into OpenLab, but we can't seem to make it work. Any help?