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GPC Slice Settings

Question asked by sridharkam on Dec 20, 2019
Latest reply on Jan 17, 2020 by sridharkam

This is a query with respect to Iron Sucrose Injection USP Monograph Identification C. Molecular
Weight Determination. The GPC calculation involves setting the Slice time in seconds. We are getting different
values of weight-average molecular weight (MW) with different slice time settings. For examples if we give 50
seconds we get 73284Da and if we give 4 seconds we get 44344Da. The limit is between 34,000–60,000 Da.
The monograph does not provide any requirement on this. Please guide us on how to choose the Slice time
setting. This is not related to peak integration. The peak integration is auto-integrated as per HPLC algorithm.