I want to determine the amounts of some compounds based on another compound that is safer and more stable. Call this compound, "Y". So I have a good working calibration curve for Y and I have determined the relative response factor of X to Y, so that I can use Y's calibration curve for X. In practice, though, I get a message that X is not quantifiable when Y is absent. Why should Y need to be present as its calibration curve is in place? I think I am doing something simple, wrong. When I attempt to view Y's calibration curve, I am told "Peak not identified" which is true, but somewhere that calibration curve should still exist.
I will restandardize Y in case its calibration curve did not make it (or was removed from) the master method.
External standard on OLCDS 2.2 client-server