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E coli sample prep for LCMS

Question asked by annlarson47 on Nov 8, 2019
Latest reply on Dec 19, 2019 by annlarson47

This question concerns sample preparation for LCMS. I am preparing an extract of peptides from Ecoli (ATCC 25922) for identification by LCMS per the following reference: PMID: 26510657, J. Am. Soc. Mass Spectrom. (2016) 27:194-210. After cell growth, the sample is centrifuged, washed, and then resuspended in 70% formic acid. Due to the non-volatility of formic acid, we are having difficulty drying the sample. We don't have a lyophilizer, so do you have any simple ideas we could try to get us through this point? We can make any modifications to the prep to achieve the desired result of extracted peptides. Best regards.