I am using 1260 prime with 6470A LC/MS/MS for the determination of NPEO and OPEO in textile samples, I need some help regarding the quantification of APEO’s (NPEO 2-18+ OPEO 2-16).
I prepared the calibration curve of individual isomer of each NPEO and OPEO using mass hunter 10.0 quantitation software.
But I have below concern.
1-For APEO concentration calculation, I need compound group analysis which mean APEO = NEPO’s (2-18) + OPEO (2-16).
2-Weight fraction calculation and fitting in curve of NPEO’s and OPEO’s in calibration curve.
3- Individual Sum of all NPEO (2-18) and OPEO’s (2-16).
So please share the guideline (Software pictures) step by step for Group amount calculation .
FYI I am following the ISO 18254 and Agilent below method.
Agilent ASMS 2018 Poster ThP-174