Can anyone provide an background information on how the Gaussian smoothing in the Spectral Options -> Mass Spectra window works?
More specifically how would you go about selecting a justifiable width value?
I have a lot of noise in my mass spectrum which is obviously affecting the quality of the data and smoothing will be appropriate. I work in a regulated environment though so anything which affects the appearance of the raw data (like smoothing) needs to be well justified.
I'm using Openlab Chemstation Edition C.01.07 SR1 to control a single quad 61xx MSD. My analytes are quadruply charged and weigh several thousand Da.