I'd like to inject a very concentrated sample and use split to avoid overloading the system.
In short, I need to create a calibration curve for a highly volatile compound, and a diluted sample would mean large solvent peak that would swallow the peak of interest. I thus thought of making a high-concentration mixture and using a 1:20 split, which would give me exactly the right absolute amount of the compound of interest going to the column but get rid of 95% of the solvent.
But before I inject such a concentrated sample, I wanted to verify that I understand the solvent vent configuration correctly.
I'd appreciate it if someone who knows the system can confirm that the following configurations would really give me a 1:20 split. I highlight what I think are the most relevant numbers:
Front PTV Inlet He
Mode Solvent Vent
Pressure On 6.4845 psi
Total Flow On 9 mL/min
Septum Purge Flow On 3 mL/min
Gas Saver On 20 After 4 min mL/min
Purge Flow to Split Vent 95 mL/min at 0.01 min
Vent Flow 100 mL/min per min
Vent Pressure 7.36 psi Until 0.01 min
Thanks in advance!