Is it normal to see the peaks broadening after flushing with isopropanol? This is the second time we flushed the column with isopropanol. The first time also gave the same peak results and therefore we changed to a new column. Now with the new column, we tried to clean the column again with isopropanol and then we run our sample with our solvent and the peaks broaden. Any suggestion on how to solve this? We are using:
Column: Phenomenex Luna 3um silica,
Mobile phase: Hexane:acetone
Thanks for your help.