I added tags to this post to increase visibility. I am also going to move this post to the Liquid Chromatography section where it is more likely to find someone able to answer your question.
I believe for some HPLC detectors it is possible to save the uncorrected signal without the reference. What type of detector are you using?
Thanks for your reply!
The detector is G1315B or G1315D, Chemstation C01.08 or C01.09.