Aslam o Alaikum ( may peace and blessing upon all of you)
My C18 column seems saturated as there is elevation of pressure and ghost peaks. I am using Agilent zorbax C18 (150* 4.6 mm 5um ) in quality control of pesticides. Is it possible to regenrate column? If yes, then your suggestions please
Attached is the Column User Guide for Zorbax column. You may want to refer to the "Cleaning Your Column" section for regeneration. Hope it will work for your column.
Hope this help.