hi there. i'm using agilent qualitative analysis B.07.00. mass hunter. i am trying to be as precise as possible about quantifying a drug in samples and minimizing background. i try selecting for the specific range for EIC but still have some background in my no drug control. i am wondering what the best most specific way is to integrate and quantify. thank you!
Hi ChemBio20 - we would actually recommend using MassHunter Quantitative Analysis for doing quantitative work. It's more directly suited to doing what you're looking to do.
In general - if you have response of an EIC signal in your blank runs - there are two approaches to handling it:
1.) You have real carry over caused by inadequate flushing of your chromatographic system. (needle wash, column rinsing, etc) might be needed.
2.) You can subtract the response of the blank runs from your samples (Quant does this, Qual does not).
Hope this helps.
Best,
Hexatriene
Hello Chembio20,
Do you need further assistance with your request or the suggestions from Hexatriene worked out for you?
Let me know.
Best regards,
Rejean
Hello,
I added tags to this post to increase visibility.