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Normal phase to Reverse phase method development

Question asked by gemma.chapman on Feb 21, 2019
Latest reply on Feb 22, 2019 by noreuter

I work in a university analytical lab. I have a Thermo Dionex uHPLC that we run in reverse phase. I currently have a student who wants to determine the purity of some NMPA he has synthesised using a Zorbax C18 column; and then establish the raecemic ratios on a chiral column (Chirobiotic T). The paper he is working from gives him a normal phase method using a mobile phase of 50:50 hexane:IPA. I would rather not have to swap our system to normal phase for one project thus I would like to see if it is possible to change the method to use reverse phase solvents. I have little experience in HPLC method development, and none in converting between normal and reverse phase solvents. Can anybody help? What reverse phase solvents and ratios can I substitute for 50:50 hexane: IPA? Or any other starting suggestions would be appreciated. Thanks.