I had to convert from Empower to Chemstation and finding it quite difficult.
I am trying to set up a calibration method for my compound which elutes as three separate peaks (different isoforms). I am using a 6-point standard curve with the reference standard. I defined three peaks as a separate compounds and grouped them together defining a total amount in the sample for each level. What I get is a separate standard curve for each compound.
How can I add all the peaks to generate a standard curve for a total amount (consisting of three peaks)?
Also, in the reporting I would like to still see what is the percentage of each of the compounds for the assessment of the quality of my product.
I would be really thankful for any hints.